DCNmodel/cnmodel/mechanisms/ampa_trussell.mod

TITLE Model of AMPA receptors

COMMENT
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    Kinetic model of AMPA receptors
    ===============================

    6-state gating model:
    (scheme 1 from Raman and Trussell, Neuron 9:173-186, 1992)
    2 open states provide dual exponential response.

               O1
               |
    C -- C1 -- C2 -- O2
               |
               D

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    This mod file does not include mechanisms for the release and time course
    of transmitter; it is to be used in conjunction with a separate mechanism
    to describe the release of transmitter and that provides the concentration
    of transmitter in the synaptic cleft (to be connected to pointer C here).

    Default parameters are set for a miniature EPSC.

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    Code based on Destexhe's ampa5.mod

    B. Graham, Dept. of Computing Science & Maths, University of Stirling
    (Contact: b.graham@cs.stir.ac.uk)
    (previously IANC, Division of Informatics, University of Edinburgh)

    CNS 2000 Version (19/11/02)

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    Further modified:

    Paul Manis (Otolaryngology/HNS and Cell and Molecular Physiology,
    UNC Chapel Hill. contact: pmanis@med.unc.edu)

    3/15/2005 Modifications:

    1. Added Q10/qfac to allow temperature scaling. All rates in the state model
    are changed by the same factor. A Q10 of 1.5 gives a decay tau (single
    exponential fit using Praxis algorithm in NEURON; using ampa_kinetics.hoc)
    of about 850 usec at 22 deg C and 570 usec at 33 deg C. These are consistent
    with the Raman and Trussell 1992 measurements in avians. The 850 usec is a
    bit fast for an EPSC, and could probably be tuned by adjustment of some of
    the parameters below.

    2. Brought several variables out to global (rather than range) so that we
    can change them - Q10 and gmax in particular. note that gmax is in pS. Only
    local conductance etc. is in specified as RANGE.

    3. Max open probability is less than unity, so a gmax of 2500 yields 100 pA
    at -60 mV. Therefore scaling by mini size must take this into account.

    3/28/2005 Paul B. Manis
    Added rectification to AMPA R. Rectification is controlled by
    polyamine-style block of receptor. See Donevan and Rogawski, 1995; Washburn
    et al., 1997. The equations used here are from Washburn et al. The values
    given in the equation at the break point were determined from EPSCs in 5
    21-d old DBA mice. Blocker = 45 (uM), Kd = 31.32, zd = 1.029. Note that this
    should also reduce the maximal conductance. Mode: if 1, use rectifying; if
    0, use non-rectifying. Default is 1

    This point process uses XMTR as the transmitter concentration to operate on
    the receptor kinetics. XMTR should be provided by another process that
    controls release (e.g., COH calyx of Held, etc). An advantage of this is
    that whatever release process is present, glutamate accumulates in the
    cleft, and can drive desensitization etc.

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ENDCOMMENT

INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}

NEURON {
THREADSAFE
    POINT_PROCESS AMPATRUSSELL
    POINTER XMTR
    RANGE C0, C1, C2, D, O1, O2

    RANGE Rb, Ru1, Ru2, Rd, Rr, Ro1, Rc1, Ro2, Rc2, Open, MaxOpen
    GLOBAL vmin, vmax
	GLOBAL Q10, Mode
	GLOBAL zd, Kd0
    RANGE g, rb, gmax, PA, Erev
    NONSPECIFIC_CURRENT i
}

UNITS {
    (nA) = (nanoamp)
    (mV) = (millivolt)
    (pS) = (picosiemens)
    (umho) = (micromho)
    (mM) = (milli/liter)
    (uM) = (micro/liter)
}

PARAMETER {

    Erev    = 7    (mV) : reversal potential
    gmax    = 10  (pS)  : maximal conductance
    vmin = -120 (mV)
    vmax = 100  (mV)
	Q10 = 1.5 : temperature sensitivity
    Mode = 0 : flag to control rectification calculation

: polyamine block parameters (Wang & Manis unpublished data)
	zd = 1.032     
	PA = 45
	Kd0 = 31.e-6

: Rates

    Rb  = 13   (/mM /ms): binding
                : diffusion limited (DO NOT ADJUST)
    Ru1 = 0.3  (/ms)    : unbinding (1st site)
    Ru2 = 200  (/ms)    : unbinding (2nd site)
    Rd  = 30.0   (/ms)  : desensitization (WAS30.0)
    Rr  = 0.02 (/ms)    : resensitization
    Ro1 = 100    (/ms)  : opening (fast)
    Rc1 = 2    (/ms)  : closing
    Ro2 = 2    (/ms)   : opening (slow)
    Rc2 = 0.25    (/ms) : closing

    Open = 0 (1) : total of all open states
    
    : Maximum open probability with Mode=0 (no rectification). 
    : This is determined empirically by holding XMTR at a large
    : value for 100 timesteps and measuring the maximum value
    : of Open.
    MaxOpen = 0.72418772400 (1)  
    
	aflag = 1 : Flag for control of printout of initial values.....

}

ASSIGNED {
    v       (mV)    : postsynaptic voltage
    i       (nA)    : current = g*(v - Erev)
    g       (pS)    : conductance
	g0		(pS)	: conductance for voltage-dependent block by polyamines
	gvdep   (pS)   : voltage-dependence of conductance
    XMTR    (mM)    : pointer to glutamate concentration
    rb      (/ms)   : binding
	qfac   : q10 factor for rate scaling
    celsius (degC)

}

STATE {
    : Channel states (all fractions)
    C0      : unbound
    C1      : single glu bound
    C2      : double glu bound
    D       : single glu bound, desensitized
    O1      : open state 1
    O2      : open state 2
}

INITIAL {
    usetable = 0
	C0=1
    C1=0
    C2=0
    D=0
    O1=0
    O2=0
    Open = 0
	qfac = Q10^((celsius-22)/10)
:	VERBATIM
:	fprintf(stdout, "AMPA.MOD gmax: %f    Q10 = %f  celsius = %f\n", gmax, Q10, celsius);
:    ENDVERBATIM
    gvdepcalc(v)
}

BREAKPOINT {
    SOLVE kstates METHOD sparse
 :   VERBATIM
 :   fprintf(stderr, "kstates @ t=%7.2f Rb: %f  XMTR: %f:  gmax: %f, o1: %f   o2: %f\n", t, Rb, XMTR, gmax, O1, O2);
 :   ENDVERBATIM

    gvdepcalc(v)
    Open = O1 + O2
    g = gmax * Open / MaxOpen
    if ( Mode == 1) {
		g0 = 1.0 + 0.6*exp((v-50)/40)  : eq. 5 of Washburn et al., 1997, slightly modified
		gvdep = g0*(1/(1+PA/(Kd0*exp(-zd*v/25.3))))
		i = (1e-6) * g * gvdep * (v - Erev)
	}
	else {
		i = (1e-6)*g*(v-Erev)
	}
}

KINETIC kstates {

    rb = Rb * XMTR

    ~ C0 <-> C1 (rb*qfac,Ru1*qfac)
    ~ C1 <-> C2 (rb*qfac,Ru2*qfac)
    ~ C2 <-> D  (Rd*qfac,Rr*qfac)
    ~ C2 <-> O1 (Ro1*qfac,Rc1*qfac)
    ~ C2 <-> O2 (Ro2*qfac,Rc2*qfac)
    CONSERVE C0+C1+C2+D+O1+O2 = 1
}

LOCAL g0
PROCEDURE gvdepcalc(v) {
	TABLE gvdep DEPEND PA, Kd0, zd FROM -100 TO 100 WITH 200
 :   VERBATIM
 :   fprintf(stderr, "gvdepcalc starts ");
 :   ENDVERBATIM
    g0 = 1.0 + 0.6*exp((v-50)/40)  : eq. 5 of Washburn et al., 1997, slightly modified
    gvdep = g0*(1/(1+PA/(Kd0*exp(-zd*v/25.3))))
  :  VERBATIM
  :  fprintf(stderr, "& ends\n");
  :  ENDVERBATIM
}